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KMID : 1024520020110030177
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Journal of the Environmental Sciences
2002 Volume.11 No. 3 p.177 ~ p.182
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Optimal Culture Conditions for Production of Environment-Friendly Biosurfactant by Pseudomonas sp. EL-G527
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Cha Mi-Sun
Lim Eun-Gyoung
Lee Keun-Hee
Cho Sun-Ja
Son Hong-Joo
Lee Sang-Joon
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Abstract
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A biosurfactant-producing microorganism was isolated from activated sludge by enrichment culture when grown on a minimal salt medium containing n-hexadecane as a sole carbon source. This microorganism was identified as Pseudomonas sp. and it was named Pseudomonas sp. EL-G527. It’s optimal culture condition is 2% n-hexadecane, 0.2% NHNO, 0.3% KHPO, 0.3% HPO, 0.02% MgSOㆍ7, 0.0025% CaCkㆍ6, 0.0015% FeSOㆍ7 in 1 distilled water and initial pH 7.0. Cultivation was initiated with a 2% inoculum obtained from starter cultures grown in 30 of the same medium in 250 flask. They were cultivated at 3 in reciprocal shaking incubator and the highest biosurfactant production was observed after 4 days.
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KEYWORD
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Pseudomonas sp. EL-G527, biosurfactant, activated sludge, n-hexadecane
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